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《Biocompatible ionic liquid enhances transdermal antigen peptide delivery and preventive vaccination effect - Tahara et al. - Unknown - U》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
SupplementaryinformationforBiocompatibleionicliquidenhancestransdermalantigenpeptidedeliveryandpreventivevaccinationeffectYoshiroTahara,1,†,‡KahoMorita,1,†RieWakabayashi,1,3NorihoKamiya,1,2,3andMasahiroGoto1,2,3,*1DepartmentofAppliedChemistry,GraduateSchoolofEngineering,2CenterforFutureChemistry,and3CenterforAdvancedTransdermalDrugDeliverySystemCenter,KyushuUniversity,744Motooka,Nishi-ku,Fukuoka819-0395,Japan*Correspondingauthor,e-mail:m-goto@mail.cstm.kyushu-u.ac.jp.†Theseauthorscontributedequally.‡Presentaddress:FacultyofScienceandEngineering,DoshishaUniversity,1-3TataraMiyakodani,KyotanabeCity,Kyoto610-0321,JapanS1
1FigureS1.ChemicalstructureandtheresultofMALDI-TOF-MSof(A)peptide(SIINFEKL)and(B)FITC-peptide(FITC-β-Ala-SIINFEKL).S2
2FigureS2.(Continued)S3
3FigureS2.1H-NMRspectrumof(A)[Cho][C8]ind6-DMSO,(B)[Cho][C10]ind6-DMSO,(C)[Cho][C12]ind6-DMSO,(D)[Cho][C14]ind6-DMSO,(E)[Cho][C16]ind6-DMSO,(F)[Cho][C18:0]inCDCl3and(G)[Cho][C18:1]ind6-DMSO.S4
4FigureS3.CellviabilityofDC2.4cellstreatedwith[Cho][FA]andcontrolsamplesfor24-h.N=3.FigureS4.PseudoternaryphasediagramofIL,EtOHandIPM.S5
5FigureS5.Crosssectionofmouseskinafter4htreatmentofFITC-peptideinEtOH/PBS,EtOH/IPMandIL/EtOH/IPM.FigureS6.FITRspectraofthelipidregionforSC.S6
6FigureS7.Bodyweightofmicefromoneweekaftertwiceimmunizationatoneweekinterval.SamplesandtumorinoculationaresameasFigure7.N=6.FigureS8.Immunehistochemicalevaluationofpeptideuptakebylangerin-positiveantigenpresentingcellsintheskin.Blue:DAPI(nuclei).Green:FITC-peptide.Red:PE-labeledanti-langerin(CD207)antibody.S7
7TableS1ElementalAnalysisof[Cho][C18:1][Cho][C18:1]CHNFound69.68%11.96%3.37%Calc.for[Cho][C18:1]-H2O68.44%12.24%3.47%TableS2Meltingpointsof[Cho][FA].aND:notdetectableduetoitslowstability.bRef:G.KnotheandR.O.Dunn,J.Am.OilChem.Soc.,86,843–856(2009).ILsMeltingpoint[°C]FattyacidsMeltingpoint[°C]b[Cho][C8]NDaCaprylicacid(C8)16.5[Cho][C10]10Capricacid(C10)31.5[Cho][C12]22Lauricacid(C12)44[Cho][C14]28Myristicacid(C14)58[Cho][C16]43Palmiticacid(C16)63[Cho][C18:0]56Stearicacid(C18:0)71.2[Cho][C18:1]-27Oleicacid(C18:1)16.3TableS3SkinpermeationparameterscalculatedfromtheresultofFigure5A(N=3).aParametersofEtOH/PBSwerecalculatedfromonesamplebecausethetwodataofthreesamplesofEtOH/PBSwerelowandhardtocalculate.NS:notsignificant,***p<0.001,comparedwithEtOH/IPM.EtOH/PBSEtOH/IPMIL/EtOH/IPMFluxJ(µgcm-2h-1×10-2)1.03a3.19±1.6828.7±2.9***LagtimetaNSlag(h)2.93.01±0.322.54±0.11DiffusioncoefficientD(cm2h-1×10-5)9.54a9.35±0.2911.1±0.5NSPartitioncoefficientK(-)133a136±641062±73***S8
